Axenic Culture of Myxamoebae of the Myxomycete Physarum polycephalum
نویسندگان
چکیده
منابع مشابه
Growth of large plasmodia of the myxomycete Physarum polycephalum.
A method has been developed for growing Physarum polycephalum plasmodia that are 8 to 10 times larger than those obtained in the petri dish cultures used by Nygaard, Guttes, and Rusch. In the large-scale procedure, plasmodia were grown in metal trays on a membrane supported by filter paper on stainless-steel screen. Plasmodia were started from a ring of inoculum to allow inward and outward migr...
متن کاملMass Culture of a Slime Mold, Physarum Polycephalum.
The slime mold, Physarum polycephalum, was cultivated in a soluble natural medium in shake flasks and in 30-liter and 50-gal conventional baffled fermentors. Yields of 6 to 10 g (dry weight) per liter were obtained in the large-scale fermentations. Because of the slow growth of the myxomycete, particular attention had to be paid to aseptic technique. The inability of this organism to withstand ...
متن کاملChange in properties of Physarum polycephalum amoebae during extended culture.
During two years of passage, several myxamoebal strains of Physarum polycephalum changed with regard to their properties in crosses and plaque morphology. These changes have been correlated with increased nuclear deoxyribonucleic acid content.
متن کاملLogical Modelling of Physarum Polycephalum
In the paper we proposed a novel model of unconventional computing where a structural part of computation is presented by dynamics of Plasmodium of Physarum polycephalum, a large single cell. We sketch a new logical approach combining conventional logic with process calculus to demonstrate how to employ formal methods in design of unconventional computing media presented by Physarum polycephalu...
متن کاملNuclear DNA content and chromosome numbers throughout the life cycle of the Colonia strain of the myxomycete, Physarum polycephalum.
Nuclear DNA content and ploidy have been determined at different stages of the life cycle of the Colonia strain of the myxomycete Physarum polycephalum. Analyses at the plasmodial stage showed that (a) Burton and Fuelgen DNA analyses agreed within 15% with strains which ranged from 0-6 to 3-6 pg of DNA per nucleus; (b) S-phase in Colonia is during the early part of interphase as in the Wisconsi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1972
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.111.1.242-247.1972